Metkinen Chemistry - reagents for DNA and RNA synthesis Metkinen Chemistry - Reagents for RNA and DNA synthesis.
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6-SIMA phosphoramidite

[(3,6-dipivaloyl-2,7-diphenyl-4,7-dichlorofluoresceinyl)-6-carboxamidohexyl]-1-O- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite



Catalogue number: 303-55

SIMA is a full spectral analog of HEX in all fluorescent applications, but has significantly improved stability. It has a higher quantum yield and molecular extinction than HEX. SIMA is also more versatile in oligo synthesis especially due to improved stability during deprotection and simple purification of labeled oligos

Formula:: C58H64Cl2N3O10P
Molecular Weight: 1065.0

Purity: > 95%

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. SIMA labeled oligos are stable at 55C in ammonium hydroxide (up to 6-8 hours) and can be deprotected with AMA.

Storage: Freezer storage, -10 to -30C, dry.

Stability in Solution: 2-3 days, <90% efficient after 4 days

Store labeled oligo in the dark, either dry or in a neutral aqueous media at -20C. Do not store crude fluorescently labeled oligonucleotides in ammonia.



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